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Title   랫드의 전암성 병변인 간 효소변이소에서 성장분획표지의 경시적 변화 ( Sequential Changes of Proliferative Fraction of Enzyme Altered Foci in Experimental Rat Hepatocarcinogenesis )
Publicationinfo   2000 Jan; 032(03): 563-571.
Key_word   Proliferative activity , Gultathione S Transferase - placeutal form , Enzyme altered foci , Hepatoma model
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Abstract   Purpose: The proliferative activity of cells in enzyme altered foci of the rat hepatoma model was measured by double immunohistochemical staining methods using anti-bromodeoxyuridine (BrdU) and anti-glutathione S transferase of placental form (GST-P). The aim of this study was to compare the cell proliferative activity in GST-P positive altered foci and in negative foci. Materials and Methods: Eight-week-old male Sprague-Dawley rats were administered by 200 mg/kg diethylnitrosamine (DEN) intraperitoneally, and followed by 0.02% acetylaminofluorene (AAF)-containing diet for 4 weeks. One week after administration of AAF diet, two-thirds hepa-tectomy was performed. Control animals were treated as same except for the omission of AAF in the diet. The rats were sacrified 0, 1, 3, 5, 7, 14 and 21 days after partial hepatectomy. The slices of liver were fixed in acetone, dehydrated in benzene and stained by peroxidase-anti-peroxidase method against GST-P and by avidine-biotin peroxidase complex method against BrdU. Results: The area of the GST-P positive foci was increased during the experimental period. In the experimental group, the S-phase fraction in the foci remained high during the first week and was decreased thereafter. However, the GST-P negative area maintained a low S-phase cell fraction throughout the experimental period. Conclusion: These results suggest that hepatic cells in the enzyme altered foci may escape a suppressor effect of AAF in contrast to the normal cells in which their growth are inhibited by AAF.
저 자   김우호(Woo Ho Kim),유은실(Yun Sil Yoo),김용일(Yong Il Kim),이미숙(Mi Sook Lee),이민재(Min Jae Lee),장자준(Ja June Jang)